Kaempferol Regresses Carcinogenesis through a Molecular Cross Talk Involved in Proliferation, Apoptosis and Inflammation on Human Cervical Cancer Cells, HeLa

Kaempferol, a flavonoid, contains plethora of therapeutic properties and has demonstrated its efficacy against cancer. This study aims to unravel the molecular targets that are being modulated by kaempferol on HeLa cells. Various assays were performed, namely: MTT assay, flow cytometry analyze DNA content quantitate apoptosis. Quantitative PCR protein profiling performed evaluate manifestation different genes involved in apoptosis, cell growth inflammation. Kaempferol exhibited reduction viability cells (IC50 = 50 µM 48 h), whereas it did not show any significant effect AC-16 line. Kaempferol-impacted apoptosis was definitive, as induced fragmentation, caused disruption membrane potential, accumulation G2-M phase augmented early Consistently, modulating expression various at both transcript levels. It upregulated pro-apoptotic genes, including APAF1, BAX, BAD, Caspases 3, 9, etc., level Bad, Bax, p27, p53, p21, 3 8 etc. level, while downregulated pro-survival gene BCL-2, BIRC8, MCL-1, XIAP, NAIP Bcl-2, Livin, clap-2 level. attenuated oxidative stress upregulating GSH activity anti-inflammatory response suppressing NF-kB pathways. Moreover, averted rampant division AKT/MTOR MAP kinase Hence, can be considered natural agent with differential profile.